Skip to main content
Fig. 1 | BMC Cell Biology

Fig. 1

From: ULK1 phosphorylates Sec23A and mediates autophagy-induced inhibition of ER-to-Golgi traffic

Fig. 1

ULK1 interacts with Sec23A and affects ERES morphology. a Sec23A but not TRAPPC12 was pulled down by ULK1 in co-immunoprecipitation experiment. Myc-ULK1, FLAG-FIP200 were co-transfected with the indicated GFP fusion DNA constructs. Myc-ULK1 was immunoprecipitated with anti-c-Myc antibody and the presence of GFP-Sec23A or GFP-TRAPPC12 was determined. b ULK1 affects the morphology of ERES. Myc-ULK1 wild type or kinase dead (KD) mutant were co-transfected with GFP-Sec23A into Hela cells. ULK1 transfected cells were determined by immunostaining of TRITC conjugated anti-Myc antibody. ERES was detected with Sec31A staining. Asterisks in the Merge pictures indicate cells transfected with ULK1

Back to article page