Fig. 4
From: Adenylyl cyclase mRNA localizes to the posterior of polarized DICTYOSTELIUM cells during chemotaxis
ACA mRNA localization at the posterior of migrating cells requires 3′ ACA cis-acting elements. a Schematic of the ACA-5’ORF or ACA-3’ORF constructs and the location of specific ACA primers used in these experiments (black arrows). b RT-PCR of the ACA-5’ORF or ACA-3’ORF cell lines using specific ACA primers. c Representative merged phase contrast and maximum intensity confocal fluorescent images depicting DAPI (blue) and ACA mRNA (pink) in ACA-5′ ORF/aca − and ACA-3′ ORF/aca − cells. d The simulated estimate of ACA mRNA units across cells is plotted for ACA-5′ ORF/aca − and ACA-3′ ORF/aca − cells. The boxes show the 50% confidence region from the median (black dashed line). The bars cover a region with 99% confidence level from the median. The statistical significance was inferred by the t-test: *** represents p < 0.01 (n = 10–15)