TY - JOUR AU - Koul, Mytre AU - Kumar, Ashok AU - Deshidi, Ramesh AU - Sharma, Vishal AU - Singh, Rachna D. AU - Singh, Jasvinder AU - Sharma, Parduman Raj AU - Shah, Bhahwal Ali AU - Jaglan, Sundeep AU - Singh, Shashank PY - 2017 DA - 2017/07/20 TI - Cladosporol A triggers apoptosis sensitivity by ROS-mediated autophagic flux in human breast cancer cells JO - BMC Cell Biology SP - 26 VL - 18 IS - 1 AB - Endophytes have proven to be an invaluable resource of chemically diverse secondary metabolites that act as excellent lead compounds for anticancer drug discovery. Here we report the promising cytotoxic effects of Cladosporol A (HPLC purified >98%) isolated from endophytic fungus Cladosporium cladosporioides collected from Datura innoxia. Cladosporol A was subjected to in vitro cytotoxicity assay against NCI60 panel of human cancer cells using MTT assay. We further investigated the molecular mechanism(s) of Cladosporol A induced cell death in human breast (MCF-7) cancer cells. Mechanistically early events of cell death were studied using DAPI, Annexin V-FITC staining assay. Furthermore, immunofluorescence studies were carried to see the involvement of intrinsic pathway leading to mitochondrial dysfunction, cytochrome c release, Bax/Bcl-2 regulation and flowcytometrically measured membrane potential loss of mitochondria in human breast (MCF-7) cancer cells after Cladosporol A treatment. The interplay between apoptosis and autophagy was studied by microtubule dynamics, expression of pro-apoptotic protein p21 and autophagic markers monodansylcadaverine staining and LC3b expression. SN - 1471-2121 UR - https://doi.org/10.1186/s12860-017-0141-0 DO - 10.1186/s12860-017-0141-0 ID - Koul2017 ER -