Fig. 11

Cladosporol A induces autophagy in human breast cancer (MCF-7 cells) (a). The autophagic vacuoles were observed under fluorescence microscope (40×) with MDC staining. The treatment of Cladosporol A and BEZ235 (positive control group) induced concentration-dependent formation of autophagic vacuoles in MCF-7 cells after 24 h. b Detection of autophagy with LC3b antibody using confocal microscopy. Immunocytochemical staining was conducted using anti-LC3b antibody and Alexa Flour-555-labelled secondary antibody. Nuclei were stained with DAPI. c Detection of autophagy by western blot analysis after Cladosporol A treatment in concentration dependent manner