Fig. 5

a Cell cycle distribution in human breast cancer (MCF-7) cells treated with 5, 10, 20 μM of Cladosporol A. Data are expressed as the mean ± SD of three similar experiments. b Cladosporol A disrupts the microtubules of human breast cancer (MCF-7) cells. Cells were treated with indicated concentrations of Cladosporol A for 24 h. Immunocytochemical staining was conducted using anti α-tubulin antibody and Alexa Flour-555-labelled secondary antibody and nuclei were stained with DAPI (c). Analysis of p21 protein expression of human breast cancer (MCF-7) cells after 24 h of Cladosporol A (5, 10, 20 μM) treatment by immunofluorescence microscopy (d). Western blot indicating the increase in p21 protein expression after Cladosporol A treatment in concentration dependent manner