Fig. 8

Cladosporol A induces loss of mitochondrial transmembrane potential. Human breast cancer (MCF-7) cells were incubated with different concentrations of Cladosporol A for 24 h. Thereafter, cells were stained with Rh-123 (1 μM) for 20 min and analyzed by (a) Flow cytometer (b). Confocal microscopy (c)). Histogram showing the effect of Cladosporol A on Δψm measured with laser scanning confocal microscope by staining with Rhodamine 123. Data are presented as mean ± S.D., statistical analysis was done with **p < 0.01