Fig. 9

Colocalization of cytochrome c and mitochondria was determined by confocal microscopy. Human breast cancer (MCF-7) cells were immunostained for cytochrome c release (green) and the mitochondria of the cells were stained with MitoTracker (red). MCF-7 cells were treated with different concentrations of Cladosporol A and doxorubicin (500 nM) for 24 h and stained with anti-cytochrome c antibody and Alexa Fluor 488-labeled secondary antibody