Fig. 1

STAU2 is differentially regulated through the cell cycle in hTert-RPE1 cells. a hTert-RPE1 cells were synchronized by a double thymidine block (DTB) or a nocodazole arrest (Ndz) followed by shake off (S.off). Cells were then released in a fresh medium for different time periods as indicated (Rel (h)). Asynchronous (As) cells were collected as controls. Protein extracts from synchronized cells were analyzed by SDS-PAGE and western blotting to investigate STAU2 pattern migration and expression of cell cycle markers (MPM2 and cyclins). β-actin was used as loading control. As control of synchronization, the percentage of cell population in the G₁, S or G₂/M phases was determined by FACS analysis (n = 3)(see also Additional file 1). b STAU2 migration dynamics was examined in hTert-RPE1 cells. Cells were blocked in prometaphase with nocodazole (Ndz) without shake-off, released in fresh medium and harvested every 15 min (Rel (min)). Extracts from untreated asynchronous (As) and nocodazole-treated cells were analyzed by western blotting. The percentage of cell population within the G₁, S or G₂/M phases was determined by FACS (n = 3). In both (a) and (b), Western blots are representatives of three independently performed experiments that showed similar profiles. Error bars represent the standard deviation