Fig. 2
From: The M-phase specific hyperphosphorylation of Staufen2 involved the cyclin-dependent kinase CDK1
Post-translational modification of STAU2 in mitosis. a hTert-RPE1 and HeLa cells were synchronized in mitosis with either nocodazole (Ndz) or paclitaxel (Taxol) and enriched by shake off (S.off). Protein extracts from synchronized cells were analyzed by SDS-PAGE and western blotting to investigate STAU2 migration pattern and expression of cell cycle markers (MPM2 and cyclins). β-actin was used as loading control. b As control of synchronization, the percentage of cell population in the G1, S or G2/M phases was determined by FACS analysis (n = 3). c HeLa cells were synchronized either in prometaphase by nocodazole (Ndz), in G1/S transition by double-thymidine block (DTB) or in late G2 by the CDK1 inhibitor RO-3306 (RO-3306) and released from the block for the indicated time periods to reach mitosis. Protein extracts from synchronized cells were analyzed by SDS-PAGE and western blotting to investigate STAU2 migration pattern and expression of mitotic markers (MPM2 and cyclins). β-actin was used as loading control. d As control of synchronization, the percentage of cell population in the G1, S or G2/M phases was determined by FACS analysis (n = 3). Error bars represent the standard deviation