Fig. 5

STAU2 is phosphorylated in the tubulin-binding domain during mitosis. a Schematic representation of STAU2 deletion mutants. Dark gray boxes, double-stranded RNA-binding domains (dsRBD); light gray boxes, tubulin-binding domain (TBD); white boxes, yellow fluorescent protein (YFP) or red fluorescent protein (mCherry). b hTert-RPE1 cells were infected with retrovirus expressing either STAU2^N-ter-FLAG₃, STAU2^52-C-ter-YFP or STAU2^59-C-ter-mCherry and protein extracts were analyzed by western blotting to detect STAU2 phosphorylation pattern migration (anti-STAU2, anti-FLAG, anti-GFP and anti-RFP) and expression of mitotic markers (p-S28-H3). β-actin was used as loading control. Western blots are representatives of three independently performed experiments that showed similar profiles