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Fig. 6 | BMC Cell Biology

Fig. 6

From: The M-phase specific hyperphosphorylation of Staufen2 involved the cyclin-dependent kinase CDK1

Fig. 6

Multiple residues are phosphorylated in the TBD during mitosis. a Schematic representation of STAU2 showing the 4 double-stranded mRNA-binding domains (dsRBD) and the tubulin-binding domain (TBD). Large-scale mass spectrometry experiments previously identified ten amino acid residues that are specifically phosphorylated during the M-phase. Three, seven and ten residues were simultaneously mutated to generate phospho-mimetic (P(3)+, P(7)+, P(10)+) and phospho-null (P(3)-, P(7)-, P(10)-) STAU252-FLAG3 and STAU259-FLAG3 mutants in the retroviral pMSCV vector. b,c hTert-RPE1 were infected with viruses expressing empty pMSCV, wild type (WT) or phospho-STAU252-FLAG3 (b) or phospho-STAU259-FLAG3 (c) mutants and selected with puromycin. Asynchronous (−) and mitotic (+)(Ndz + S.off) cell extracts were analyzed by western blotting to detect STAU2 migration and mitotic markers (MPM2 and p-S28-H3). All western blots are representatives of three independently performed experiments that showed similar profiles

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