Fig. 7
From: The M-phase specific hyperphosphorylation of Staufen2 involved the cyclin-dependent kinase CDK1
STAU2 is phosphorylated by Cdk1 during mitosis in hTert-RPE1 cells. a hTert-RPE1 cells were incubated with nocodazole (Ndz) for 16 h in the absence (DMSO) or presence of specific kinase inhibitors for 4 h as indicated. Asynchronous (As) cells were used as controls. Cells were treated with the proteasome inhibitor MG132 (+) to keep cells in mitosis. STAU2 migration on SDS-gels was analyzed by western blotting. Cell markers were used to confirm specific inhibition of kinases. b Cell synchronization was confirmed by FACS. Western blots and FACS are representatives of three independently performed experiments that showed similar profiles