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Fig. 1 | BMC Cell Biology

Fig. 1

From: EphA receptors and ephrin-A ligands are upregulated by monocytic differentiation/maturation and promote cell adhesion and protrusion formation in HL60 monocytes

Fig. 1

M-CSF induces differentiation of non-adherent bone marrow MNCs to adherent cells of the monocytic lineage. a NSE staining of mouse bone marrow MNCs immediately after fractionation and of adherent MNCs from the MC-1d group (non-adherent MNCs cultured in medium containing 20 ng/mL M-CSF that adhered within one day) and MC-5d group (non-adherent MNCs cultured in medium containing 20 ng/mL M-CSF that adhered within two days and were cultured for three more days). b RT-PCR amplification of the myeloid cell marker CD34, and the monocyte marker CD115 in bone marrow MNCs from the MC-1d and MC-5d group. Densitometric quantification of mRNA expression levels from three independent experiments, normalized to GAPDH, is shown as the means ± SD. The expression of CD115 in the MC-5d group is upregulated 2.80-fold (P = 0.002) compared to that in the MC-1d group, whereas CD34 expression levels are almost the same between both groups

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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