Fig. 10

Representative images of F-actin and vinculin highlighting HL60 cell morphology in the VD-TNF group. HL60 cells from the VD-TNF group were cultured on a coverslip surface on which the regions adsorbed with EphA2-Fc or ephrin-A1-Fc and Fc plus Matrigel and those with Fc plus Matrigel appeared alternatively as stripes. Fluorescence images of F-actin staining (red) and vinculin immunostaining (green) highlighting morphology of cells on the Fc and Matrigel-adsorbed control surface (Con), the EphA2-Fc and Fc plus Matrigel-adsorbed, and the ephrin-A1-Fc and Fc plus Matrigel-adsorbed test surfaces were photographed. a Cells extend wider and frequently show a number of large protrusions accompanied with prominent F-actin on the Fc-chimera-protein-adsorbed test surfaces. b Prominent dot-like focal adhesions labeled with anti-vinculin antibody were accompanied by spots of F-actin in the cells on the EphA2-Fc-adsorbed surface. Doughnut-shaped focal adhesions accompanied with F-actin in their cores frequently appeared in cells on the ephrin-A1-Fc-adsorbed surface