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Fig. 2 | BMC Cell Biology

Fig. 2

From: EphA receptors and ephrin-A ligands are upregulated by monocytic differentiation/maturation and promote cell adhesion and protrusion formation in HL60 monocytes

Fig. 2

RT-PCR amplification of EphA and ephrin-A mRNAs from mouse bone marrow MNCs treated with M-CSF. a MC-1d represents non-adherent MNCs cultured in medium containing 20 ng/mL M-CSF that adhered within one day, and MC-5d represents non-adherent MNCs cultured in medium containing 20 ng/mL M-CSF that adhered in two days and were cultured for three more days. Compared to other EphAs and ephrin-As, EphA2 and ephrin-A1 required one less cycle of amplification. b Densitometric quantification of mRNA expression levels from three independent experiments, normalized to GAPDH, is shown as the mean ± SD. The expression levels of EphA2, ephrin-A1, and ephrin-A2 in the MC-5d group were upregulated by 2.10, 3.66, and 2.56-fold, respectively (P = 0.014, P = 0.011, P = 0.001) compared to that in the MC-1d group, whereas EphA7 expression was downregulated in the MC-5d group and was less than the detection limit after 38 cycles of PCR amplification

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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