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Fig. 5 | BMC Cell Biology

Fig. 5

From: EphA receptors and ephrin-A ligands are upregulated by monocytic differentiation/maturation and promote cell adhesion and protrusion formation in HL60 monocytes

Fig. 5

RT-PCR amplification of EphA and ephrin-A mRNAs in HL60 cells. HL60 cells were treated with the vehicle (control group; Con), 50 nM VD (VD group; VD), and 50 nM VD with 5 ng/mL TNFα (VD-TNF group; VD-TNF). a Treatments with VD and/or TNFα induce EphA2 and EphA4 expression in HL60 cells. b Densitometric quantification of mRNA expression levels from three independent experiments, normalized to GAPDH, is shown as the mean ± SD. The expression level of EphA1 was similar among the three groups whereas that of ephrin-A4 in the VD-TNF group was elevated 2.08-fold and was significantly higher than that in the control (P = 0.039)

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