Fig. 8

HL60 cells treated with VD and TNFα preferentially occupy the EphA2-Fc or ephrin-A1-Fc-adsorbed surface. a A schematic drawing illustrating the procedure of Fc-chimera protein adsorption to the coverslip surface in stripes. A comb-shaped silicon mask was applied to the coverslip surface. The Fc-chimera or Fc proteins were adsorbed onto the surface. After washing, the mask was removed. Subsequently, Fc followed by Matrigel was adsorbed onto the glass surface. After washing, the coverslips were placed in 6 cm culture dishes with culture medium. HL60 cells were plated and cultured for 16 h. b Typical phase-contrast micrographs showing HL60 cells treated with VD and TNFα (VD-TNF group) cultured on the coverslip surface wherein regions adsorbed with EphA2-Fc or ephrin-A1-Fc appeared as stripes. In the control stripes using Fc instead of the chimera proteins, HL60 cells of the VD-TNF group did not form stripes with different cell densities. Native HL60 cells (Con) scarcely adhere to an ephrin-A1-Fc-adsorbed surface. c Quantified cell densities in regions adsorbed with EphA2-Fc or ephrin-A1-Fc and Fc plus Matrigel compared to those with Fc and Matrigel in HL60 cells from the VD-TNF group. The results from three independent experiments are shown. Data are presented as means ± SD. **P < 0.01