Table 2 Primers and cycle numbers for RT-PCR amplification of human mRNAs
Human | Primer | Product size (bp) | Annealing temp. (°C) | Cycle number | |
|---|---|---|---|---|---|
CD14 | Forward | 5’-AGAACCTTGTGAGCTGGACGAT-3’ | 369 | 61.0 | 24 |
Reverse | 5’-GAAAGTGCAAGTCCTGTGGCTT-3’ | ||||
CD15 | Forward | 5’-TTTGGATGAACTTCGAGTCGCC-3’ | 360 | 60.0 | 30 |
Reverse | 5’-AAGCCAGGTAGAACTTGTAGCG-3’ | ||||
CD16 | Forward | 5’-GACTGAAGATCTCCCAAAGG-3’ | 668 | 52.3 | 24 |
Reverse | 5’-CCTTCCAGTCTCTTGTTGAG-3’ | ||||
CD115 | Forward | 5’-GTGGTAGAGAGTGCCTACTT-3’ | 342 | 53.4 | 30 |
Reverse | 5’-CCATATGACGCTTACCTCTG-3’ | ||||
integrin α1 | Forward | 5’-GTCTATCCACGGAGAAATGG-3’ | 417 | 50.0 | 32 |
Reverse | 5’-CTCACAGAGTCCTGAAAGTC-3’ | ||||
integrin α2 | Forward | 5’-CTACAATGTTGGTCTCCCAG-3’ | 440 | 50.0 | 32 |
Reverse | 5’-CAACATCTATGAGGGAAGGG-3’ | ||||
integrin α4 | Forward | 5’-GATCATCTTACTGGACTGGC-3’ | 320 | 54.8 | 32 |
Reverse | 5’-CAGATCTGAGAAGCCATCTG-3’ | ||||
integrin α5 | Forward | 5’-CCAGCCCTACATTATCAGAG-3’ | 390 | 50.0 | 32 |
Reverse | 5’-GAGATGAGGGACTGTAAACC-3’ | ||||
integrin α6 | Forward | 5’-GGAGATAAACTCCCTGAACC-3’ | 416 | 50.0 | 32 |
Reverse | 5’-CGAGAATAGCCACTAGGATG-3’ | ||||
integrin αD | Forward | 5’-ATCAGCAGGCAGGAAGAATC-3’ | 518 | 50.1 | 32 |
Reverse | 5’-ACCTCGTCTTCTTCTAGCAC-3’ | ||||
integrin αL | Forward | 5’-GCCCTGGTTTTCAGGAATG-3’ | 430 | 53.0 | 32 |
Reverse | 5’-CCAATCCCGATGATGTAGC-3’ | ||||
integrin αM | Forward | 5’-GTGTGATGCTGTTCTCTACG-3’ | 364 | 50 | 32 |
Reverse | 5’-CTCCATGATTGCCTTGACTC-3’ | ||||
integrin αX | Forward | 5’-CCAGATCACCTTCTTGGCTAC-3’ | 523 | 61.3 | 32 |
Reverse | 5’-CTTCAGGGTGAAATCCAGCTC-3’ | ||||
integrin β1 | Forward | 5’-TTCAAGGGCAAACGTGTGAG-3’ | 459 | 54.4 | 28 |
Reverse | 5’-CCGTGTCCCATTTGGCATTC-3’ | ||||
integrin β2 | Forward | 5’-CAATAAACTCTCCTCCAGGG-3’ | 522 | 52.5 | 28 |
Reverse | 5’-CAGTACTGCCCGTATATCAG-3’ | ||||
Rap1A | Forward | 5’-GTACAAGCTAGTGGTCCTTG-3’ | 371 | 50.0 | 29 |
Reverse | 5’-CAACTACTCGCTCATCTTCC-3’ | ||||
EphA1 | Forward | 5’-TGGTGGAACTTCCTTCGAGA-3’ | 446 | 61.2 | 35 |
Reverse | 5’-ACAATCCCAAAGCTCCACAC-3’ | ||||
EphA2 | Forward | 5’-CGCCGGCTCTGATGCACCTT-3’ | 312 | 62.0 | 38 |
Reverse | 5’-TCCTGAGGTGCCCGGAAGAA-3’ | ||||
EphA3 | Forward | 5’-TGCGGTCAGCATCACAACTA-3’ | 380 | 59.3 | 38 |
Reverse | 5’-TTGCTACTGCCGCTGAAATG-3’ | ||||
EphA4 | Forward | 5’-TGCAGCTTTTGTCATCAGCC-3’ | 381 | 60.3 | 38 |
Reverse | 5’-TTAGTGACCACGCCTTCCAA-3’ | ||||
EphA5 | Forward | 5’-CTCTGGACGTGCCTTCTC-3’ | 319 | 56.6 | 38 |
Reverse | 5’-TCCCCAGTCCTCCAGGAA-3’ | ||||
EphA6 | Forward | 5’-TGCGAAGTCCGGGAATTTCT-3’ | 627 | 61.6 | 38 |
Reverse | 5’-ACGAACAGTGAAGGGGCATT-3’ | ||||
EphA7 | Forward | 5’-AAAGCTGACCAAGAAGGCGA-3’ | 311 | 62.0 | 38 |
Reverse | 5’-TCAAACTGCCCCATGATGCT-3’ | ||||
EphA8 | Forward | 5’-ATCACCTACAATGCCGTGTG-3’ | 341 | 58.4 | 38 |
Reverse | 5’-TACTCCAGGATGATGCCGTT-3’ | ||||
EphA10 | Forward | 5’-CACGTACCAAGTGTGCAATG-3’ | 646 | 56.8 | 38 |
Reverse | 5’-CAACTGGATACCTTCGCAGA-3’ | ||||
ephrin-A1 | Forward | 5’-GGAACCCAGACCCATAGGAG-3’ | 825 | 59.7 | 38 |
Reverse | 5’-CCCGTTTTGAGGCTGCTAGG-3’ | ||||
ephrin-A2 | Forward | 5’-TACGTGCTGTACATGGTCAACG-3’ | 316 | 58.1 | 38 |
Reverse | 5’-GGCTGCTACACGAGTTATTGCT-3’ | ||||
ephrin-A3 | Forward | 5’-TTTACTGCCCGCACTACAACAG-3’ | 523 | 59.7 | 38 |
Reverse | 5’-AACGTCATGAGGAAGAAGGCGA-3’ | ||||
ephrin-A4 | Forward | 5’-GCCCCGAGACGTTTGCTTTGTA-3’ | 364 | 62.5 | 34 |
Reverse | 5’-TTGTCGGTCTGAATTGGCACCC-3’ | ||||
ephrin-A5 | Forward | 5’-ACTCTCCAAATGGACCGCTGAA-3’ | 363 | 66.0 | 38 |
Reverse | 5’-TCAAAAGCATCGCCAGGAGGAA-3’ | ||||
GAPDH | Forward | 5’-GTCGGAGTCAACGGATTTGG-3’ | 607 | 57.2 | 21 |
Reverse | 5’-GGATGATGTTCTGGAGAGCC-3’ | ||||