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Fig. 2 | BMC Cell Biology

Fig. 2

From: Honokiol improved chondrogenesis and suppressed inflammation in human umbilical cord derived mesenchymal stem cells via blocking nuclear factor-κB pathway

Fig. 2

Determination of multi-differentiation of hUC-MSCs. 2nd passage hUC-MSCs were used for multi-differentiation determination. For osteogenesis or adipogenesis, cells were cultured in 6-wells plate with osteogenic or adipogenic medium for 2 weeks. Chondrogenesis was conducted in pellet culture for 2 weeks (n = 6). a: control group. b: Chondrogenesis group cells. Hematoxylin-eosin and alcian blue dual-staining (arrow indicated the positive alcian blue staining). c: Osteogenesis group cells (arrow indicated the positive von Kossa staining). d: Adipogenesis group cells (arrow indicated the positive oil red O staining). The results showed here were representative of all samples from 6 donors. The expression of differentiation related genes was evaluated by qRT-PCR. e: Chondrogenesis related genes. f: Osteogenesis related genes. g: Adipogenesis related genes. Data was analyzed by using the 2-ΔΔCT method. All qRT-PCR results were presented as mean ± SD (n = 9); p* < 0.01 versus control

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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