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Table 1 Differentiation culture medium

From: Honokiol improved chondrogenesis and suppressed inflammation in human umbilical cord derived mesenchymal stem cells via blocking nuclear factor-κB pathway

Osteogenesis Low glucose a-MEM (Gibco, USA)
10% (v/v) fetal bovine serum (Gibco, USA)
0.1uM dexamethasone (Sigma-Aldrich, USA)
10 mM β-glycerolphosphate (Sigma-Aldrich, USA)
0.2 mM ascorbic acid (Sigma-Aldrich, USA)
Chondrogenesis Low glucose a-MEM (Gibco, USA)
0.1 uM dexamethasone (Sigma-Aldrich, USA)
50 μg/mL AsA, 100 μg/mL sodium pyruvate (Sigma-Aldrich, USA)
40 μg/mL proline (Sigma-Aldrich, USA)
10 ng/mL TGF-1 (Invitrogen, USA)
50 mg/mL ITS+ premix (Becton Dickinson, USA)
Adipogenesis Low glucose a-MEM (Gibco, USA)
0.5 mM 3-isobutyl-1-methylxanthine (IBMX, Sigma-Aldrich, USA)
1 μM hydrocortisone (Sigma-Aldrich, USA)
0.1 mM indomethacin (Sigma-Aldrich, USA)
10% rabbit serum (Sigma-Aldrich, USA)