Table 2 Primer sequences for qRT-PCR
Gene | Forward sequence 5′-3′ | Reverse sequence 5′-3’ |
|---|---|---|
GADPH | TGTTGCCATCAATGACCCCTT | CTCCACGACGTACTCAGCG |
ALP | CCACGTCTTCACATTTGGTG | AGACTGCGCCTGGTAGTTGT |
RUNX-2 | AGTGGACGAGGCAAGAGTTTC | CCTTCTGGGTTCCCGAGGT |
SOX-9 | CTTCCGCGACGTGGACAT | GTTGGGCGGCAGGTACTG |
Aggrecan | ACAGCTGGGGACATTAGTGG | GTGGAATGCAGAGGT GGTTT |
Col2α1 | GCCTGGTGTCATGGGTTT | GTCCCTTCTCACCAGCTTTG |
CEBP | AGGAACACGAAGCACGATCAG | CGCACATTCACATTGCACAA |
FABP4/aP2 | TACTGGGCCAGGAATTTGAC | GGACACCCCCATCTAAGGTT |
Caspase-3 | AGAACTGGACTGTGGCATTGAG | GCTTGTCGGCATACTGTTTCAG |
MMP-1 | AGTGACTGGGAAACCAGATGCTGA | GCTCTTGGCAAATCTGGCCTGTAA |
MMP-9 | GCGGAGATTGGGAACCAGCTGTA | GACGCGCCTGTGTACACCCACA |
MMP-13 | TGCTGCATTCTCCTTCAGGA | ATGCATCCAGGGGTCCTGGC |