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Fig. 2 | BMC Cell Biology

Fig. 2

From: Coral cell separation and isolation by fluorescence-activated cell sorting (FACS)

Fig. 2

Coral cell differentiation patterns. P. damicornis cell suspension was incubated with different fluorescent markers and analyzed on FACS Accuri C6. All of the samples were analyzed on two dimensional plots of the green channel- 488 nm (533/30BP) and on the far-red channel-640 nm (675/25BP) to differentiate the Symbiodinium positive cells. Gated in black are the differentiated populations with their cell percentage indicated. The analysis was done after differentiation of cells and debris on FSC and SSC and live/dead cells using PI. a) Background control of unstained cells for all of the panels. In the gate are the Symbiodinium cells positive at 640 nm (675/25BP) due to natural fluorescence. b) Labeled cells with Phen Green, a color that is quenched by bi-valent metals. A small population of cells had the lowest level of metal and it differentiated from the main population. c) Labeled cells with ALDEFLUOR that indicates high levels of ALDH activity. No clear differentiation was found, but the width of the signal in different cells is 3 fluorescent log scales which enables us to differentiate low, moderate and high labeling. d) Cells containing ROS, labeled with CellRox. Clear negative and positive populations (positive marked) were identified. Note that all the Symbiodinium positive cells are positive to ROS. e) Cells were incubated with glucose analog 2-NBDG. Positive labeling was found, but no clear differentiation. f) Cells were co-incubated for 3 h with green fluorescent beads for analysis of engulfment. Cells positive to beads are marked

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