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Fig. 3 | BMC Cell Biology

Fig. 3

From: Coral cell separation and isolation by fluorescence-activated cell sorting (FACS)

Fig. 3

Examples of cell differentiation from a chemical compound screening. The P. damicornis cell suspension was incubated with the indicated chemical compounds for 30 min at 20oC and analyzed by FACS LSRFortessa. The analysis was done after differentiation of cells and debris on FSC and SSC and live/dead cells using DAPI, and separated to Symbiodinium positive cells (e–h) and Symbiodinium negative cells (a–d, as shown in Fig. 1). a) Background control of unstained Symbiodinium negative cells for (b–d). b) Labeled cells with CAY10455, had separation for 3 potential populations shown in gates. c) Labeled cells with Rhodamine Phenylglyoxal had separation of 4 potential populations. d) Labeled cells with Acridine Orange had separation of 3 populations shown in red. e) Background control of unstained Symbiodinium positive cells for (f). f) Labeled cells with Dihydroethidium had separation of 3 populations positive to Symbiodinium. g) Background control of unstained Symbiodinium positive cells for (h). h) Labeled cells with Acridine Orange had separation of 3 populations positive to Symbiodinium

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