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Table 1 Screened compounds to differentiate coral cells

From: Coral cell separation and isolation by fluorescence-activated cell sorting (FACS)

Compound Name

Company and Catalog

Fluorescent Indicator

Label

Differ. Cells (Symb.+)

Conc.

3-dodecanoyl-NBD Cholesterol

CC: 13220

Cholesterol uptake

N

N (N)

1 μM

MeOSuc-Ala-Ala-Pro-Val-AMC

CC: 14907

Elastase activity

N

N (N)

1 μM

Dihydroethidium

CC: 12013

Intracellular superoxide

Y

Y (Y)

1 μM

Pyronin Y

CC: 14488

dsRNA

Y

Y (Y)

0.2 μM

ThioFluor623

CC: 13083

Intracellular thiols

Y

Y (Y)

1 μM

Rhodamine Phenylglyoxal

CC: 16172

Protein citrullination

Y

Y (Y)

0.2 μM

CAY10455

CC: 10005072

Arachidonoyl ethanolamide uptake

Y

Y (N)

0.2 μM

Dibenzylfluorescein

CC: 16808

Cytochrome P450 activity

Y

N (Y)

0.2 μM

BCECF-Acetoxymethyl ester

CC: 15922

Intracellular pH

Y

Y (Y)

0.2 μM

NBD-FTY720 phenoxy HCl

CC: 16841

Sphingosine-1-phosphate

Y

N (N)

1 μM

10-Acetyl-3,7-dihydroxyphenoxazine

CC: 10010469

Hydrogen Peroxide/Peroxidase

Y

N (N)

1 μM

2-NBDG

CC: 11046

Glucose uptake

Y

N (N)

1 μM

1-NBD-decanoyl-2-decanoyl-sn-Glycerol

CC: 9000341

Diacylglycerol uptake

Y

N (N)

1 μM

(Z-Ala-Ala-Ala-Ala)2Rh110

CC: 11675

Elastase activity

N

N (N)

1 μM

5-Cyano-2,3-di-(p-tolyl)tetrazolium Cl

CC: 15926

Aerobic metabolic activity

N

N (N)

1 μM

Coumarin Boronic Acid pinacolate ester

CC: 10818

Peroxynitrite

N

N (N)

1 μM

Garcinol

CC: 10566

Histone acetyltransferase inhibitor

N

N (N)

1 μM

WSP-1

CC: 11179

Hydrogen Sulfide

N

N (N)

1 μM

Zinpyr-1

CC: 15122

Intracellular Zinc ion

Y

N (Y)

0.2 μM

DPPP

CC: 62237

Hydroperoxides

N

N (N)

1 μM

Acridine Orange

CC: 14338

DNA dye

Y

Y (Y)

0.2 μM

Fura-PE3-Acetoxymethyl ester

CC: 15366

Intracellular Calcium

Y

Y (N)

0.2 μM

Lucigenin

CC: 14872

Intracellular Chloride ion

Y

N (Y)

1 μM

CONA-Lectin

TFS: C21402

Binds α-man, α-glc

Y

Y (N)

2 μg/ml

EUA-Lectin

SA: L8262

Binds α-L-fuc and (glcNAc)2

Y

N (N)

2 μg/ml

PNA-Lectin

SA: L7381

Binds β-gal(1 → 3)galNAc

Y

Y (Y)

2 μg/ml

WGA-Lectin

TFS: W32465

Binds sialic acid and Nac

Y

Y (N)

2 μg/ml

pHrodo Red

TFS: P35372

Intracellular pH indicator

Y

Y (lables)

5 μM

LysoTracker Deep Red

TFS: L12492

Acidic organelles

Y

Y (lables)

0.2 μM

CellROX green

TFS: C10492

Reactive oxygen species

Y

Y (lables)

1 μM

Phen Green

TFS: P6763

Heavy metal indicator

Y

Y (lables)

1 μM

ALDAFLUOR

ST: 1700

ALDH-bright cells

Y

Y (lables)

0.4 μg/ml

DAPI

TFS: D1306

AT regions of DNA

Y

Y (Y)

3 μM

PI

TFS: R37108

DNA

Y

Y (N)

5 μg/ml

  1. P. damicornis cell suspensions were incubated with indicated chemical compounds and analyzed by FACS. Companies used: CC Cayman Chemical, TFS Thermo Fisher Scientific, SA Sigma-Aldrich, ST Stemcell Technologies. If the chemical compound is fluorescently labeling cells it is indicated as Y (Yes) and if the result is the same as the control it is indicated as N (No) in the label column. If the labeling can clearly differentiate cellular populations by the level of labeling it is indicated as Y in the differentiation column of cells, differentiation capability of cells that are positive to Symbiodinium indicated in scopes. The concentration used throughout the study are indicated in the last column