Fig. 5

Increased proliferation of senescent cell populations following resveralogue treatment. a Cell numbers of NHDF, MRC5 and HF043 fibroblast populations following treatment with resveralogues 1–6. Experiments were carried out in triplicate for three biological replicates and *** represents p < 0.001 (2 way ANOVA). b Proliferation index was assessed for control and treated NHDFs, as well as younger (PD25) cells as assessed by Ki67 immunofluorescence (> 400 nuclei counted per sample, *** p < 0.001 by 2 way ANOVA). c Telomere length was quantified by qPCR relative to the 36B4 endogenous control and normalised to telomere length in vehicle-only controls, younger passage cells (PD25) and in cells treated with compounds 1–6. Experiments were carried out in triplicate for three biological replicates. Statistical significance is indicated by * = p < 0.05, ** = p < 0.005, *** = p < 0.0005 (2 way ANOVA)