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Fig. 7 | BMC Cell Biology

Fig. 7

From: Reduction of pluripotent gene expression in murine embryonic stem cells exposed to mechanical loading or Cyclo RGD peptide

Fig. 7

Tri-lineage marker analysis in the presence of cRGDfC, w/ and w/o LIF. Early ectodermal (a,b), mesodermal (c,d) and endodermal (e,f) markers were examined within the different treatment groups by quantitative PCR. (***;p < 0.0001; **;p < 0.001; *;p < 0.05). Flow cytometry validation of PCR data (e-i). Representative data from 3 independent experimental replicates. These results were confirmed using flow cytometry in the Oct-4 negative cell population of each treatment group (g-j)

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