Skip to main content
Fig. 2 | BMC Cell Biology

Fig. 2

From: A novel interaction between kinase activities in regulation of cilia formation

Fig. 2

AurA and Nek2 activity is additive when cilia are growing. ARPE-19 cells were transfected with the following plasmids or combinations: AurA, Nek2, AurA/Nek2, AurAKD, Nek2KD, AurAKD/Nek2KD (AKD/NKD). Transfected cells were grown for 24 h then cilia assembly was induced by serum starvation (assembling, left panels) followed by reintroduction of serum to induce cilia disassembly (disassembling, right panels). Cilia number (a) and length (b) were measured for a total of 300 cells. Significant differences are indicated with similar symbols; p ≤ 0.05. AurA/Nek2 and AKD/NKD are significantly different from all other transfectants. (c) Cells transfected with either empty vector (top) or the AKD/NKD combination (bottom) were stained for gamma tubulin in green, acetylated tubulin in red, and DNA in blue. Scale bar equals 10 μm. The means for cilia number and length ± SEM are shown in Table 1

Back to article page