Fig. 3
From: A novel interaction between kinase activities in regulation of cilia formation
Nek2 and AurA form a complex in ARPE-19 cells. (a) Cells were cotransfected with plasmids expressing AurA and Nek2 and cell lysates immunoprecipitated with either AurA antibodies (lane 1), Nek2 antibodies (lane 2), or no antibody (land 3) and probed for either AurA or Nek2. (b) Untransfected ARPE-19 cell lysates were immunoprecipitated with either AurA (lane 1), Nek2 (lane 2), or no antibody and probed for either AurA or Nek2. Each immunoprecipitate was blotted for the immunoprecipitating antibody to verify immunoprecipitation