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Fig. 7 | BMC Cell Biology

Fig. 7

From: A novel interaction between kinase activities in regulation of cilia formation

Fig. 7

PPP1R42 depletion reduces cilia number and length and produces abnormal cilia. (a) ARPE-19 cells were treated with either PPP1R42 targeting siRNA (knockdown, KD) or with off-target siRNA (off target, OT) and grown for 24 h (cycling, left panel), then cilia assembly was induced by serum starvation (assembling, middle panel) following reintroduction of serum to induce cilia disassembly (disassembling, right panel). Cilia number and length (a and b) were measured in each condition, 300 cells were counted. Significant differences are indicated with similar symbols; p ≤ 0.05. Percentage distorted cilia is shown by the red bars in panel A. (c-e) ARPE-19 cells were treated with R42 KD siRNA for 24 h and three representative cells are shown with cilia stained green with anti-acetylated tubulin antibody (enriched in cilia) and nuclei blue with DAPI. Insets show deformed cilia at higher magnification. (f) A representative control cell treated with off-target siRNA. Scale bar = 10 μm. The means for cilia number and length ± SEM are shown in Table 1

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