Fig. 8
From: A novel interaction between kinase activities in regulation of cilia formation
PPP1R42 interacts with Nek2 and inhibits Nek2 activity in vitro. (a) PPP1R42 was overexpressed with either Nek2 or AurA in ARPE-19 cells, grown for 24 h (C, cycling) then the cells were induced to assemble cilia (SS, serum starved) followed by disassembly (+S, serum added back). Protein complexes were immunoprecipitated with anti-PPP1R42 antibody, or no antibody as negative control (−C), and the blots probed for Nek2 or AurA. Immunoprecipitates were treated with λ-phosphatase and probed with Nek2 antibody (a, right panel). (b) Protein complexes were immunoprecipitated from untransfected ARPE-19 cells with anti-AurA and anti-Nek2 antibodies and probed for PPP1R42. (c) Nek2 kinase activity was assayed in the presence of 50, 150 and 300 nM recombinant R42 protein and activity calculated as a percentage of untreated Nek2 activity