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Fig. 9 | BMC Cell Biology

Fig. 9

From: A novel interaction between kinase activities in regulation of cilia formation

Fig. 9

AurA and Nek2 counteract the stimulatory effect of PPP1R42 on cilia. ARPE-19 cells coexpressing PPP1R42 (R42) with either kinase (R42/Nek2; R42/AurA), or expressing either kinase alone were grown for 24 h (cycling, left panel), then cilia assembly was induced by serum starvation (assembling, middle panel) following by reintroduction of serum to induce cilia to disassemble (disassembling, right panel). Transfection with empty vector served as negative control. Cilia number (a) and length (b) were measured at each condition; 300 cells were counted. Significant differences are indicated with asterisks; p ≤ 0.05. The means for cilia number and length ± SEM are shown in Table 1

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