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Table 1 Summary of percentage cilia and length for transfections

From: A novel interaction between kinase activities in regulation of cilia formation

  Percentage cilia Length, μm
  Cycling Assemb Disassemb Cycling Assemb Disassemb
Empty 1.7 ± 0.8 42.1 ± 3.7 12.6 ± 1.4 1.7 ± 0.4 4.1 ± 0.1 2.9 ± 0.1
AurA 0 26.5 ± 2.8 8.8 ± 0.9 0 4.1 ± 0.1 3.1 ± 0.2
Nek2 0.5 ± 0.5 27.8 ± 2.3 7.2 ± 1.9 0.9 ± 0.5 4.2 ± 0.1 3.0 ± 0.1
AurA/Nek2 ND 13.3 ± 1.0 15.5 ± 1.6 ND 7.2 ± 0.2 7.0 ± 0.3
AurAKD ND 31.7 ± 1.8 13.6 ± 1.3 ND 4.5 ± 0.2 4.0 ± 0.1
Nek2KD ND 32.2 ± 1.2 16.8 ± 1.8 ND 5.3 ± 0.2 3.2 ± 0.2
AurAKD/Nek2KD ND 34.5 ± 2.7 25.6 ± 1.5 ND 14.0 ± 0.8 10.1 ± 0.3
AurA/Nek2KD ND 12 ± 1.3 18 ± 2.0 ND 3.9 ± 0.1 3.3 ± 0.1
AurAKD/Nek2 ND 11 ± 1.0 19.6 ± 2.0 ND 3.4 ± 0.2 3.0 ± 0.1
R42 9.1 ± 1.3 47.2 ± 2.9 22.3 ± 3.0 4.2 ± 0.3 4.3 ± 0.1 3.4 ± 0.2
R42/AurA 1 ± 0.4 24.8 ± 2.1 7.2 ± 1.0 2.6 ± 0.7 4.3 ± 0.1 2.7 ± 0.1
R42/Nek2 0.8 ± 0.4 18.4 ± 2.6 12.5 ± 2.6 2.2 ± 0.8 4.0 ± 0.1 3.0 ± 0.1
OT siRNA 4.8 ± 1.3 46 ± 4.6 26.3 ± 8.6 2.6 ± 0.2 4.0 ± 0.1 2.9 ± 0.1
R42 siRNA 3.4 ± 0.7 41 ± 2.2 24 ± 4.0 2.7 ± 0.2 3.6 ± 0.1 3.4 ± 0.2
Empty-MLN ND 26.4 ± 3.1 13.2 ± 1.3 ND 6.4 ± 0.4 5.3 ± 0.4
Empty + MLN ND 33.4 ± 1.7 18.3 ± 3.3 ND 6.8 ± 0.4 4.8 ± 0.4
Nek2-MLN ND 25.2 ± 2.3 10.6 ± 1.5 ND 5.9 ± 0.4 4.8 ± 0.4
Nek2 + MLN ND 36.2 ± 2.0 17.5 ± 1.4 ND 6.3 ± 0.3 4.8 ± 0.4
Empty-rac ND 46 ± 3.7 17.6 ± 2.0 ND 4.6 ± 4.7 5.3 ± 0.6
Empty + rac ND 43 ± 5.8 27.7 ± 2.3 ND 10.8 ± 1.2 6.4 ± 0.6
AurA-rac ND 33 ± 4.4 14.3 ± 2.3 ND 5.3 ± 0.7 3.8 ± 0.3
AurA + rac ND 48.4 ± 5.6 28 ± 5.0 l ND 7.4 ± 0.9 11.8 ± 1.3
  1. The mean percentage of cilia number and length ± SEM is shown for each transfection