Table 1 Summary of percentage cilia and length for transfections
From: A novel interaction between kinase activities in regulation of cilia formation
|  | Percentage cilia | Length, μm | ||||
|---|---|---|---|---|---|---|
| Â | Cycling | Assemb | Disassemb | Cycling | Assemb | Disassemb |
Empty | 1.7 ± 0.8 | 42.1 ± 3.7 | 12.6 ± 1.4 | 1.7 ± 0.4 | 4.1 ± 0.1 | 2.9 ± 0.1 |
AurA | 0 | 26.5 ± 2.8 | 8.8 ± 0.9 | 0 | 4.1 ± 0.1 | 3.1 ± 0.2 |
Nek2 | 0.5 ± 0.5 | 27.8 ± 2.3 | 7.2 ± 1.9 | 0.9 ± 0.5 | 4.2 ± 0.1 | 3.0 ± 0.1 |
AurA/Nek2 | ND | 13.3 ± 1.0 | 15.5 ± 1.6 | ND | 7.2 ± 0.2 | 7.0 ± 0.3 |
AurAKD | ND | 31.7 ± 1.8 | 13.6 ± 1.3 | ND | 4.5 ± 0.2 | 4.0 ± 0.1 |
Nek2KD | ND | 32.2 ± 1.2 | 16.8 ± 1.8 | ND | 5.3 ± 0.2 | 3.2 ± 0.2 |
AurAKD/Nek2KD | ND | 34.5 ± 2.7 | 25.6 ± 1.5 | ND | 14.0 ± 0.8 | 10.1 ± 0.3 |
AurA/Nek2KD | ND | 12 ± 1.3 | 18 ± 2.0 | ND | 3.9 ± 0.1 | 3.3 ± 0.1 |
AurAKD/Nek2 | ND | 11 ± 1.0 | 19.6 ± 2.0 | ND | 3.4 ± 0.2 | 3.0 ± 0.1 |
R42 | 9.1 ± 1.3 | 47.2 ± 2.9 | 22.3 ± 3.0 | 4.2 ± 0.3 | 4.3 ± 0.1 | 3.4 ± 0.2 |
R42/AurA | 1 ± 0.4 | 24.8 ± 2.1 | 7.2 ± 1.0 | 2.6 ± 0.7 | 4.3 ± 0.1 | 2.7 ± 0.1 |
R42/Nek2 | 0.8 ± 0.4 | 18.4 ± 2.6 | 12.5 ± 2.6 | 2.2 ± 0.8 | 4.0 ± 0.1 | 3.0 ± 0.1 |
OT siRNA | 4.8 ± 1.3 | 46 ± 4.6 | 26.3 ± 8.6 | 2.6 ± 0.2 | 4.0 ± 0.1 | 2.9 ± 0.1 |
R42 siRNA | 3.4 ± 0.7 | 41 ± 2.2 | 24 ± 4.0 | 2.7 ± 0.2 | 3.6 ± 0.1 | 3.4 ± 0.2 |
Empty-MLN | ND | 26.4 ± 3.1 | 13.2 ± 1.3 | ND | 6.4 ± 0.4 | 5.3 ± 0.4 |
Empty + MLN | ND | 33.4 ± 1.7 | 18.3 ± 3.3 | ND | 6.8 ± 0.4 | 4.8 ± 0.4 |
Nek2-MLN | ND | 25.2 ± 2.3 | 10.6 ± 1.5 | ND | 5.9 ± 0.4 | 4.8 ± 0.4 |
Nek2 + MLN | ND | 36.2 ± 2.0 | 17.5 ± 1.4 | ND | 6.3 ± 0.3 | 4.8 ± 0.4 |
Empty-rac | ND | 46 ± 3.7 | 17.6 ± 2.0 | ND | 4.6 ± 4.7 | 5.3 ± 0.6 |
Empty + rac | ND | 43 ± 5.8 | 27.7 ± 2.3 | ND | 10.8 ± 1.2 | 6.4 ± 0.6 |
AurA-rac | ND | 33 ± 4.4 | 14.3 ± 2.3 | ND | 5.3 ± 0.7 | 3.8 ± 0.3 |
AurA + rac | ND | 48.4 ± 5.6 | 28 ± 5.0 l | ND | 7.4 ± 0.9 | 11.8 ± 1.3 |