Table 5 Primer sequences used for RT-PCR validation
From: Molecular targets and signaling pathways regulated by nuclear translocation of syndecan-1
GENE | PRIMERS (5’TO 3’ORIENTATION): FORWARD/REVERSE |
|---|---|
GAPDH | ACATCATCCCTGCCTCTACTGG/ AGTGGGTGTCGCTGTTGAAGTC [99] |
SDC1 | TCTGACAACTTCTCCGGCTC/CCACTTCTGGCAGGACTACA [100] |
DOCK8 | AGTGCCGAGGACTTTGAGAA/ ATTCTGTTGCCCAGGTGTTC |
EGR1 | TGACCGCAGAGTCTTTTCCT/ TGGGTTGGTCATGCTCACTA |
NEK11 | AGAGGATGCCACATCTGACC/ GAAGTGCAACCCAGGACATT |
ZNF676 | CTGGTCTTCCTGGGTATTGC/ TTGCTCTGGCCAAAACTCTT |
CA9 | TAAGCAGCTCCACACCCTCT/ TCTCATCTGCACAAGGAACG |
COL19A1 | GTGGTTTCTGTGGCAGGTTT/AGTCTGCCTCCTCGCAATTA |
DACH1 | GTGGAAAACACCCCTCAGAA/ CTTGTTCCACATTGCACACC |
EGR2 | CCTCCTTATTCTGGCTGTGC// CTGGGATCATTGGGAAGAGA |
FAP | CTTGTCCTGGCTTCAGCTTC/ AGGTGGCAACTCCAAATACG |
HS6ST3 | GGCTCACTGAGTTCCAGAGG/ TCTAGCTGCTTGGTGTGGTG |
IL2RB | GCTGATCAACTGCAGGAACA/ TGTCCCTCTCCAGCACTTCT |
PIP5KIB | CCAGGAATGGAAGGATGAGA/ AATTGTGGTTGCCAAGGAAG |
SERPINA3 | CCAACGTGGACTTCGCTTTC/CTCTTGGCATCCTCCGTGAA |
SERPINB4 | TCAGTGAAGCCAACACCAAG/ TGTTGCAGCTTTTTCTGTGG |
TNRFS9 | CACTCTGTTGCTGGTCCTCA/ CACAGGTCCTTTGTCCACCT |
VCAM1 | CAGACAGGAAGTCCCTGGAA/ TTCTTGCAGCTTTGTGGATG |
ADAMTS5 | CCCAGCCTGGACACATTACT/ TTCCCCTGAGCATTTTTCAC |
AREG | TGGATTGGACCTCAATGACA/ AGCCAGGTATTTGTGGTTCG |
CDK20 | ATGGCTAAGGTGGCATTGTC/ CGCTCATCCTGAGGGAGTAG |
COL1A2 | CCTGGTAATCCTGGAGCAAA/ TTACCGCTCTCTCCTTTGGA |
CXCL1 | AGGGAATTCACCCCAAGAAC/ CACCAGTGAGCTTCCTCCTC |
ITGA8 | CACATTCTGGTGGACTGTGG/ AATCCCTTGTTGTTGCGTTC |
MMP10 | GGCTCTTTCACTCAGCCAAC/ GGCTCTTTCACTCAGCCAAC |
PCDH18 | AGCATCTGCAGCTTTTCCAT/ AGGGAATTTTCCCCAACATC |
SULT1B1 | GGTTATCCCATGACCTGTGC/CCAGGGAGAGTCATTTCCAA |