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Fig. 2 | BMC Cell Biology

Fig. 2

From: Mechanically tuned 3 dimensional hydrogels support human mammary fibroblast growth and viability

Fig. 2

Hydrogel properties. a Schematic of hydrogel setup and downstream assays. b Rheology was performed on hydrogels cross-linked with 20, 30 and 60 μg/ml mTG. As mTG concentration increased, the elastic modulus (G’) of the hydrogels corresponding increased. The most significant change in G’ is shown for hydrogels cross-linked with 60 μg/ml mTG. c Hydrogels cross-linked with 20, 30 and 60 μg/ml mTG were examined over a 5 h period for changes in hydrogel weight following incubation with PBS. Hydrogels cross-linked with 20 and 30 μg/ml mTG exhibited increases in hydrogel weight during the culture period indicative that the gels had swollen during this time frame. Hydrogels cross-linked with 60 μg/ml mTG exhibited a slight, but not significant decrease in hydrogel weight during the incubation period. d Differences in the dry hydrogel weight were examined for HMF encapsulated hydrogels at the start of the experiment (time 0) and at the end of the experiment (day 7). Hydrogel weights were similar between time 0 and day 7 measurements for each of the mTG hydrogels. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001

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