Fig. 1

RPMI2650 cells were treated with LPS (5 μg/mL). a CCK-8 assay was done to estimate the percentage of viable cells in LPS-treated and control group of cells; b Flow cytometry was done to assess the percentage of apoptotic cells in LPS-treated and control groups of cells; c Western blot was done to estimate different apoptosis related proteins; d LDH cytotoxicity assay was done to detect the release of LDH from LPS-treated and control group of cells; e RT-PCR was to measure relative mRNA expression of different inflammatory cytokines; f-i Elisa was done to measure exact amount of different inflammatory cytokines, namely IL-1β, IL-6, IL-8, and TNF-α. LPS: lipopolysaccharide; CCK-8 assay: cell counting kit-8; RT-PCR: quantitative real time polymerase chain reaction; Elisa: enzyme linked immunosorbent assay; IL: interleukin; TNF-α: tumor necrosis factor α.*P < 0.05; **P < 0.01;***P < 0.001