Fig. 4

Following transfection of RPMI2650 cells with miR-17-5p mimic or si-miR-17-5p. a cell viability was assessed in different groups of cells; b Percentage of apoptotic cells in different group of cells were measured by flow cytometry; c Western blot analysis was done to assess different apoptosis related factors in different groups of cells; d Relative mRNA expression of different inflammatory cytokines (IL-1β, IL-6, IL-8, and TNF-α) were assessed by RT-PCR; e-h Elisa was done to measure the exact amounts of the said inflammatory cytokines in different groups of cells. Elisa: enzyme linked immunosorbent assay; IL: interleukin; TNF-α: tumor necrosis factor α.*P < 0.05; **P < 0.01