Fig. 7

Overexpression of Smad7 protected RPMI2650 cells against LPS-induced injury. a Cell viability was significantly increased in cells overexpressing Smad7 and vice versa; b Apoptosis was also suppressed in cells overexpressing Smad7; c Western blot analysis of apoptosis related proteins (Bcl-2, Bax, cleaved-caspase-3, and cleaved-caspase-9) also showed similar findings; d RT-PCR revealed that relative mRNA expressions of the inflammatory cytokines were suppressed in cells overexpressing Smad7; e-h Elisa revealed that the amounts of the said inflammatory cytokines were decreased in cells overexpressing Smad7. LPS: lipopolysaccharide; Smad7: Mothers against decapentaplegic homolog 7; RT-PCR: quantitative real-time polymerase chain reaction. * P < 0.05