Fig. 5

The intracellular targeting of the HIV-1 Env TMD is influenced by its length. a Compared to T-E26, two amino-acids were deleted from the Env TMD in T-E28, and four in T-E30. In addition, in T-E34 the arginine present in the TMD was mutated to a leucine residue (see also Table1). b Intracellular localization of each Tac chimeric protein was determined as described in the legend to Fig. 2. Scale bar: 10 μm. c The fraction of each chimeric protein present at the cell surface was determined as described in the legend to Fig. 2. T-E30 is significantly less localized at the cell surface than T-H0 (n = 4; one-way analysis of variance: p < 0.01; *: post-hoc Tukey-Kramer p < 0.05)