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Fig. 7 | BMC Cell Biology

Fig. 7

From: Role of the HIV-1 envelope transmembrane domain in intracellular sorting

Fig. 7

The HIV-1 Env TMD associates efficiently with a TMD containing a negative charge. a To reveal a putative interaction between TMDS, HeLa cells were co-transfected with plasmids encoding the δ chain of the CD3 receptor fused to the β-galactosidase (Gal) and various Tac fusion proteins. b Tac fusion proteins were immunoprecipitated and the amount of co-precipitated β-galactosidase activity assessed to reveal the degree of association with the δ chain of the CD3 receptor. The mean ± SEM of at least five independent experiments are indicated (one-way analysis of variance: p < 0.01; *: post-hoc Tukey-Kramer p < 0.05)

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