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Fig. 5 | BMC Cell Biology

Fig. 5

From: The C-terminus of the oncoprotein TGAT is necessary for plasma membrane association and efficient RhoA-mediated signaling

Fig. 5

Effect of TGAT mutant expression on actin polymerization. a Representative images of HeLa cells transfected with YFP-TGAT or YFP-TGATΔ15 (top), stained with TRITC-phalloidin (bottom). b Quantification of F-actin in HeLa cells transfected with YFP-TGAT (n = 53), YFP-TGATC242S (n = 147), YFP-TGATC253S (n = 58), YFP-TGATC242S, C253S (n = 128), YFP-TGATΔ15 (n = 126) and stained with DAPI and TRITC-phalloidin, as determined by the fluorescent intensity of the TRITC-phalloidin staining. Actin intensity of transfected cells was normalized to the intensity of untransfected control cells in the same field of view

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