Fig. 5

Effect of TGAT mutant expression on actin polymerization. a Representative images of HeLa cells transfected with YFP-TGAT or YFP-TGAT^Δ15 (top), stained with TRITC-phalloidin (bottom). b Quantification of F-actin in HeLa cells transfected with YFP-TGAT (n = 53), YFP-TGAT^C242S (n = 147), YFP-TGAT^C253S (n = 58), YFP-TGAT^{C242S, C253S} (n = 128), YFP-TGAT^Δ15 (n = 126) and stained with DAPI and TRITC-phalloidin, as determined by the fluorescent intensity of the TRITC-phalloidin staining. Actin intensity of transfected cells was normalized to the intensity of untransfected control cells in the same field of view