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Fig. 2 | BMC Cell Biology

Fig. 2

From: Cyclic AMP signaling in Dictyostelium promotes the translocation of the copine family of calcium-binding proteins to the plasma membrane

Fig. 2

GFP-tagged copine proteins translocate to membranes in live cells treated with calcium ionophore. Cells expressing GFP or a GFP-tagged copine were treated with calcium ionophore in the presence of 2 mM CaCl2 or 5 mM CaCl2 for CpnF (Ca2+) or 5 mM EGTA (EGTA). Images were obtained with a Nikon Eclipse Ti inverted laser scanning confocal microscope using a 60× oil immersion objective. Representative images are shown for each copine cell line. Scale bar = 5 μm

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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