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Fig. 5 | BMC Cell Biology

Fig. 5

From: Cyclic AMP signaling in Dictyostelium promotes the translocation of the copine family of calcium-binding proteins to the plasma membrane

Fig. 5

Copines have different calcium-dependent and -independent membrane-binding properties. Cells expressing GFP, a GFP-tagged copine, or GST-CpnA were disrupted and all membranes were pelleted by ultracentrifugation in the presence (calcium) or absence of calcium (EGTA). The supernatant samples (S) and pelleted membranes samples (P) were analyzed by Western blot with an antibody to GFP. Three blots were analyzed by densitometry and the average percentage of the protein found in the pellet was graphed. Error bars = standard error

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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