Fig. 7
Copines vary in their binding to specific acidic phospholipids. GFP-tagged copines were isolated by immunoprecipitation and the protein/antibody complex was incubated with commercially available lipid dot blots in the presence of calcium. Binding was detected with a secondary antibody conjugated to HRP and chemiluminescence. a Multiple blots for each copine protein were analyzed and the combined data is represented as grayscale dots, with white referring to no signal observed and black referring to the most intense signal. b An image of a single dot blot showing relevant lipids bound by GFP-CpnA. Lipid abbreviations: phosphatidylserine (PS), phosphatidic acid (PA), phosphatidylinositol (PI), phosphatidylinositol 4-phosphate (PI(4)P), phosphatidylinositol 4, 5-phosphate (PI(4,5)P2) and phosphatidylinositol 3, 4,5-phosphate (PI(3,4,5)P3), triglyceride (TG), diacylglycerol (DAG), phosphatidylethanolamine (PE), phosphatidylcholine (PC), cholesterol (CH), and sphingomyelin (SM)