Fig. 8

The C2 domains of CpnF are missing key aspartates. The amino acids that make up the two C2 domains of the six Dictyostelium copines were identified using the NCBI Conserved Domain Search. The two C2 domains and intervening sequences for all the copines were aligned using Clustal Omega. BoxShade was used to indicate conserved residues within the aligned sequences. Black boxes indicate where at least five out of the six copines have the identical amino acid, while gray boxes indicate where at least five out of the six copines have a similar amino acid. Four key aspartates thought to be involved in calcium binding in each C2 domain are indicated with a number. Locations were these aspartates are missing are indicated with an X and highlighted with a red box