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Fig. 10 | BMC Cell Biology

Fig. 10

From: Mannan oligosaccharide requires functional ETC and TLR for biological radiation protection to normal cells

Fig. 10

(a-b) IR induced changes in oxidative modification of mitochondrial phospholipids (Cardiolipins). Effect of pre-irradiation treatment with mannan on NAO incorporation was measured to assess oxidative modification of cardiolipins as described under materials and methods section. (a) Radiation-induced (3 Gy) oxidation of Mitochondrial Phospholipids measured at different time intervals. (b) Effects of mannan pretreatment on radiation induced peroxidation of cardiolipin at 24 h post irradiation. Fluorescence of NAO was acquired flow cytometrically at Ex λ 488 nm and Em λ 530 nm. The data were analyzed using FlowJo V10.1 software. The data presented is representative of findings from three independent experiments

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