Fig. 7
(a-d) Changes in ROS generation and ΔΨm. Effect of IR exposure on generation of ROS and changes in ΔΨm in NKE ρ° cells (a-b) and NKE cells (c-d). ROS levels and ΔΨm were measured by flow cytometry using DHE and DiOC6(3), respectively as described in materials and method section. Histograms presented are representative of three independent experiments. Results are also expressed as fluorescence mean ± SD of three independent experiments. Differences were designated significant at values, ** p < 0.01, *** p < 0.001 and were labeled as * compared with the 3 Gy (radiation only) group. Data was analyzed using FlowJo V10.1 software