Fig. 9

(a-b) Expression of Mn SOD and Cu/Zn SOD in NKE and NKE ρ° cells. Cells were treated, harvested and samples were resolved on 8–20% gradient SDS-polyacrylamide gel followed by transfer on to PVDF membrane and blotted using specific antibodies as described in materials and method section. β-actin was used as a loading control. Results are expressed as Protein expression (fold changes) with respect to control ±SD. Differences were designated significant at values, *** p < 0.001 and were labeled as # compared with the sham irradiated control group, * compared with the 3 Gy (radiation only) group. (c) Phosphorylation of JNK and P38. To assess role of stress response pathways in mannan mediated protection activation of JNM and P38 was measured as phosphorylation status. Briefly, cells were harvested, and equal quantity of cellular proteins were resolved on 8–20% gradient SDS ployacrylamide gel, followed by transfer on to PVDF membrane and blotted using corresponding primary and secondary antibodies as described in materials and methods section. The changes in phosphorylation were compared with radiation and sham irradiation groups at different time intervals. β-actin was used as a loading control