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Fig. 1 | BMC Cell Biology

Fig. 1

From: Establishment and comparison of air-liquid interface culture systems for primary and immortalized swine tracheal epithelial cells

Fig. 1

Development of immortalized STEC line and primary STECs cultured under Ali conditions over 21 days. The immortalized STEC line and primary STECs were immersed in media, and they grew into a confluent monolayer within 2 days. At this time, the apical medium was then discarded to create Ali conditions. Cellular morphological changes at different time points (D7, D14, and D21) were observed by conventional light microscopy

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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