Fig. 2

Assessment of tight junctions in immortalized STEC line and primary STECs cultured under Ali conditions. Immortalized STEC line and primary STECs were cultured under Ali conditions for 21 days. TEER was measured on D1 and every 2 days thereafter through D21 under Ali conditions. Data are presented as resistance values (Ωcm²)and given as the mean +/− SD of three independent experiments, each done in triplicate (n = 9) (a). At D7, the cells were fixed with 4% paraformaldehyde and stained with an anti-ZO-1 antibody, followed by incubation with a Alexa Fluor 488-conjugated goat anti-mouse secondary antibody. The cells were then counterstained with DAPI and imaged using laser scanning confocal microscopy (b)