Fig. 5From: Establishment and comparison of air-liquid interface culture systems for primary and immortalized swine tracheal epithelial cellsMucus secretion by immortalized STEC line and primary STECs cultured under Ali conditions. Immortalized STEC line and primary STECs were cultured under Ali conditions for 21Â days. At D7, D14, and D21, the cells were fixed with 4% paraformaldehyde and stained with an anti-MUC5B antibody, followed by incubation with a Alexa Fluor 555-conjugated goat anti-rabbit secondary antibody. The cells were then counterstained with DAPI and imaged using laser scanning confocal microscopy (a, b)Back to article page